Enhanced Nitrite Detection by a Carbon Screen Printed Electrode Modified with Photochemically-Made AuNPs

Abstract

Excessive nitrite amounts harm the environment and put public health at high risk. Therefore, accurate and sensitive detection of nitrite in surface and groundwater is mandatory for mitigating its adverse effects. Herein, a highly sensitive electrochemical sensor based on carbon screen-printed electrodes (CSPE) surface-modified with photochemically-made gold nanoparticles (AuNPs, ~12 nm) is proposed for nitrite detection. Scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy show that AuNPs uniformly coat the CSPE, increase its surface area, and contribute to oxidizing nitrite to much lower potential (+0.5 V vs. Ag/AgCl) and faster rate. Under optimized differential pulse voltammetry conditions, the CSPE/AuNPs-PEI electrode responds linearly (R2 > 0.99) to nitrite within a wide concentration range (0.01–4.0 µM), showing a sensitivity of 0.85 µA·µM−1·cm−2 and limit of detection as low as 2.5 nM. The CSPE/AuNPs-PEI electrode successfully detects nitrite in tap water and canned water of olives, showing no influence of those matrices. In addition, the electrode’s response is highly reproducible since a relative standard deviation lower than 10% is observed when the same electrode is operated in five consecutive measurements or when electrodes of different fabrication batches are evaluated.