Bacillus thuringiensis Cyt Proteins as Enablers of Activity of Cry and Tpp Toxins against Aedes albopictus

Author:

Lai Liliana12ORCID,Villanueva Maite2,Muruzabal-Galarza Ane3,Fernández Ana Beatriz2ORCID,Unzue Argine2,Toledo-Arana Alejandro3ORCID,Caballero Primitivo1,Caballero Carlos J.2

Affiliation:

1. Institute of Multidisciplinary Research in Applied Biology-IMAB, Universidad Pública de Navarra, 31192 Mutilva, Spain

2. Departamento de Investigación y Desarrollo, Bioinsectis SL, Plaza Cein 5, Nave A14, 31110 Noáin, Spain

3. Instituto de Agrobiotecnología (IDAB), CSIC-Gobierno de Navarra, 31192 Mutilva, Spain

Abstract

Aedes albopictus is a species of mosquito, originally from Southeast Asia, that belongs to the Culicidae family and the Dipteran insect order. The distribution of this vector has rapidly changed over the past decade, making most of the temperate territories in the world vulnerable to important human vector-borne diseases such as dengue, yellow fever, zika or chikungunya. Bacillus thuringiensis var. israeliensis (Bti)-based insecticides represent a realistic alternative to the most common synthetic insecticides for the control of mosquito larvae. However, several studies have revealed emerging resistances to the major Bti Crystal proteins such as Cry4Aa, Cry4Ba and Cry11Aa, making the finding of new toxins necessary to diminish the exposure to the same toxicity factors overtime. Here, we characterized the individual activity of Cyt1Aa, Cry4Aa, Cry4Ba and Cry11Aa against A. albopictus and found a new protein, Cyt1A-like, that increases the activity of Cry11Aa more than 20-fold. Additionally, we demonstrated that Cyt1A-like facilitates the activity three new Bti toxins: Cry53-like, Cry56A-like and Tpp36-like. All in all, these results provide alternatives to the currently available Bti products for the control of mosquito populations and position Cyt proteins as enablers of activity for otherwise non-active crystal proteins.

Funder

Gobierno de Navarra

European Union’s H2020 research and innovation programme

Spanish Ministry of Science and Innovation

the Program Torres Quevedo

Bioinsectis SL

Publisher

MDPI AG

Subject

Health, Toxicology and Mutagenesis,Toxicology

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4. Tippelt, L., Werner, D., and Kampen, H. (2019). Tolerance of Three Aedes Albopictus Strains (Diptera: Culicidae) from Different Geographical Origins towards Winter Temperatures under Field Conditions in Northern Germany. PLoS ONE, 14.

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