Evaluation of the Antiseizure Activity of Endemic Plant Halfordia kendack Guillaumin and Its Main Constituent, Halfordin, on a Zebrafish Pentylenetetrazole (PTZ)-Induced Seizure Model

Author:

Skiba Adrianna1ORCID,Kozioł Ewelina1,Luca Simon Vlad23ORCID,Budzyńska Barbara4ORCID,Podlasz Piotr5ORCID,Van Der Ent Wietske6,Shojaeinia Elham6ORCID,Esguerra Camila V.67,Nour Mohammed8,Marcourt Laurence910ORCID,Wolfender Jean-Luc910ORCID,Skalicka-Woźniak Krystyna1ORCID

Affiliation:

1. Department of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, Poland

2. Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany

3. Department of Pharmacognosy, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania

4. Independent Laboratory of Behavioral Studies, Medical University, Chodzki 4a, 20-090 Lublin, Poland

5. Department of Pathophysiology, Forensic Veterinary and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland

6. Chemical Neuroscience Group, Centre for Molecular Medicine Norway (NCMM), University of Oslo, Forskningsparken, Gaustadalleén 21, 0349 Oslo, Norway

7. Department of Pharmacy, Section for Pharmacology and Pharmaceutical Biosciences, Faculty of Mathematics and Natural Sciences, University of Oslo, Blindern, P.O. Box 1068, 0316 Oslo, Norway

8. Institut des Sciences Exactes et Appliquées (ISEA)-EA 4243, France University of New Caledonia, 98851 Nouméa, New Caledonia, France

9. School of Pharmaceutical Sciences, University of Geneva, CMU, Rue Michel Servet 1, 1211 Geneva, Switzerland

10. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU, Rue Michel Servet 1, 1211 Geneva, Switzerland

Abstract

Epilepsy is a neurological disease that burdens over 50 million people worldwide. Despite the considerable number of available antiseizure medications, it is estimated that around 30% of patients still do not respond to available treatment. Herbal medicines represent a promising source of new antiseizure drugs. This study aimed to identify new drug lead candidates with antiseizure activity from endemic plants of New Caledonia. The crude methanolic leaf extract of Halfordia kendack Guillaumin (Rutaceae) significantly decreased (75 μg/mL and 100 μg/mL) seizure-like behaviour compared to sodium valproate in a zebrafish pentylenetetrazole (PTZ)-induced acute seizure model. The main coumarin compound, halfordin, was subsequently isolated by liquid-liquid chromatography and subjected to locomotor, local field potential (LFP), and gene expression assays. Halfordin (20 μM) significantly decreased convulsive-like behaviour in the locomotor and LFP analysis (by 41.4% and 60%, respectively) and significantly modulated galn, and penka gene expression.

Funder

Medical University of Lublin

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3