Affiliation:
1. National Research Council (CNR), Institute of Applied Sciences and Intelligent Systems (ISASI), Via Pietro Castellino 111, 80131 Napoli, Italy
2. CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ, 13005 Marseille, France
Abstract
Stimulated Raman scattering (SRS) microscopy is a high-speed imaging modality based on intrinsic molecular vibrations, producing chemical maps in living systems. Such capability, allowing for direct visualization without the perturbation of biological processes, has enabled a plethora of biological and medical applications. In this review, after introducing the basic theory and competitive effects of SRS, some crucial features for SRS microscopy implementations, such as noise, spectral bandwidth, speed, chemical sensitivity, spatial resolution, and quantum enhancement, are discussed. Finally, some SRS applications in biological and medical imaging are described. Even if certainly not exhaustive, we aimed to offer a broad overview, providing guidance for newcomers and hinting at a more detailed investigation to interested researchers in this rapidly growing field.
Reference134 articles.
1. Imaging without fluorescence: Nonlinear optical microscopy for quantitative cellular imaging;Streets;Anal. Chem.,2014
2. Coherent nonlinear optical imaging: Beyond fluorescence microscopy;Min;Annu. Rev. Phys. Chem.,2011
3. Meyers, R.A. (2000). Encyclopedia of Analytical Chemistry, Wiley.
4. Righini, G.C., and Sirleto, L. (2023). Woodhead Publishing Series in Electronic and Optical Materials, Advances in Nonlinear Photonics, Woodhead Publishing, Woodhead Publishing.
5. Nonlinear vibrational microscopy applied to lipid biology;Zumbusch;Prog. Lipid Res.,2013