Dehydration Treatment Improves Ulmus glabra Dormant Bud Regeneration from Cryostorage

Abstract

The conservation of genetic resources in cryocollections requires reliable protocols for the cryopreservation and the regeneration of the preserved material. With Ulmus glabra, the regeneration of thawed buds by in vitro organogenesis has suffered from low shoot growth and high contamination rates. The dehydration of the buds before cryopreservation improved the shoot growth rate and ameliorated the contamination rate of in vitro cultures initiated from thawed buds, although the degree of success varied depending on the donor tree.