Affiliation:
1. Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia
2. Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, NSW 2522, Australia
3. School of Chemistry, Monash University, Clayton, VIC 3800, Australia
4. Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
Abstract
Elevenins are peptides found in a range of organisms, including arthropods, annelids, nematodes, and molluscs. They consist of 17 to 19 amino acid residues with a single conserved disulfide bond. The subject of this study, elevenin-Vc1, was first identified in the venom of the cone snail Conus victoriae (Gen. Comp. Endocrinol. 2017, 244, 11–18). Although numerous elevenin sequences have been reported, their physiological function is unclear, and no structural information is available. Upon intracranial injection in mice, elevenin-Vc1 induced hyperactivity at doses of 5 or 10 nmol. The structure of elevenin-Vc1, determined using nuclear magnetic resonance spectroscopy, consists of a short helix and a bend region stabilised by the single disulfide bond. The elevenin-Vc1 structural fold is similar to that of α-conotoxins such as α-RgIA and α-ImI, which are also found in the venoms of cone snails and are antagonists at specific subtypes of nicotinic acetylcholine receptors (nAChRs). In an attempt to mimic the functional motif, Asp-Pro-Arg, of α-RgIA and α-ImI, we synthesised an analogue, designated elevenin-Vc1-DPR. However, neither elevenin-Vc1 nor the analogue was active at six different human nAChR subtypes (α1β1εδ, α3β2, α3β4, α4β2, α7, and α9α10) at 1 µM concentrations.
Funder
Australian Research Council
Australian National Health and Medical Research Council
Subject
Drug Discovery,Pharmacology, Toxicology and Pharmaceutics (miscellaneous),Pharmaceutical Science
Reference58 articles.
1. Robinson, S.D., Safavi-Hemami, H., McIntosh, L.D., Purcell, A.W., Norton, R.S., and Papenfuss, A.T. (2014). Diversity of conotoxin gene superfamilies in the venomous snail, Conus victoriae. PLoS ONE, 9.
2. In the picture: Disulfide-poor conopeptides, a class of pharmacologically interesting compounds;Lebbe;J. Venom Anim. Toxins Incl. Trop. Dis.,2016
3. Hormone-like peptides in the venoms of marine cone snails;Robinson;Gen. Comp. Endocrinol.,2017
4. Conotoxins as selective inhibitors of neuronal ion channels, receptors and transporters;Lewis;IUBMB Life,2004
5. Therapeutic potential of cone snail venom peptides (conopeptides);Vetter;Curr. Top. Med. Chem.,2012
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献