Abstract
Understanding chronic wound infection is key for successful treatment and requires accurate laboratory models. We describe a modified biofilm flow device that effectively mimics the chronic wound environment, including simulated wound fluid, a collagen-based 3D biofilm matrix, and a five-species mixture of clinically relevant bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, and Citrobacter freundii). Mixed biofilms were cultured for between 3 and 14 days with consistent numbers of bacteria that exhibited reduced metabolic activity, which increased with a high dose of glucose. S. aureus was recovered from biofilms as a small colony variant, but as a normal colony variant if P. aeruginosa was excluded from the system. Bacteria within the biofilm did not co-aggregate but formed discrete, species-specific clusters. Biofilms demonstrated differential tolerance to the topical antimicrobials Neosporin and HOCl, consistent with protection due to the biofilm lifestyle. The characteristics exhibited within this model match those of real-world wound biofilms, reflecting the clinical scenario and yielding a powerful in vitro tool that is versatile, inexpensive, and pivotal for understanding chronic wound infection.
Subject
Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy
Reference39 articles.
1. Omar, A., Wright, J., Schultz, G., Burrell, R., and Nadworny, P. (2017). Microbial Biofilms and Chronic Wounds. Microorganisms, 5.
2. The Health Economic Burden That Acute and Chronic Wounds Impose on an Average Clinical Commissioning Group/Health Board in the UK;Guest;J. Wound Care,2017
3. Wound Microbiology and Associated Approaches to Wound Management;Bowler;Clin. Microbiol. Rev,2001
4. Nonrandom Distribution of Pseudomonas Aeruginosa and Staphylococcus Aureus in Chronic Wounds;Fazli;J. Clin. Microbiol.,2009
5. A Systematic Review of the Effectiveness of Interventions to Enhance the Healing of Chronic Ulcers of the Foot in Diabetes;Hinchliffe;Diabetes Metab. Res. Rev.,2008
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献