Determination of Median Lethal Concentration (LC50) and Nitrite Accumulation in the Blood and Tissue of Blood Cockle (Tegillarca granosa, Linnaeus 1758)

Abstract

This study aimed to determine the nitrite toxicity of blood cockle Tegillarca granosa, with the objectives being to identify the median lethal concentration (LC50) and the accumulation level of nitrite in Tegillarca granosa, and to determine the relationship of nitrite accumulation with mortality percentage. The levels of LC50 and accumulation of nitrite were determined after 72 h of exposure to different nitrite concentrations (0, 0.5, 1.0, 1.5, and 2.0 mg/L). Nitrite accumulation was analysed using Method 8153 and a DR2800 spectrophotometer (HACH, Loveland, CO, USA). LC50 was identified at 1.53 mg/L, and nitrite accumulated in the ranges of 0.012 to 0.106 mg/L wet weight and 0.002 to 0.089 mg/L wet weight in the blood and soft tissue samples, respectively. Accumulation concentration in both tissue and blood cells increased proportionally with the exposure concentration, and had a strong positive relationship with the percentage of mortality. Our findings suggest that prolonged exposure of nitrite led to accumulation in the blood and tissues and caused cockle mortality.