Abstract
The microbiome’s significance in chronic rhinosinusitis (CRS) is unclear. Antimicrobials are recommended in acute exacerbations of the disease (AECRS). Increasing rates of antibiotic resistance have stimulated research on alternative therapeutic options, including silver nanoparticles (AgNPs). However, there are concerns regarding the safety of silver administration. The aim of this study was to assess the biological activity of tannic acid-prepared AgNPs (TA-AgNPs) towards sinonasal pathogens and nasal epithelial cells (HNEpC). The minimal inhibitory concentration (MIC) for pathogens isolated from patients with AECRS was approximated using the well diffusion method. The cytotoxicity of TA-AgNPswas evaluated using an MTT assay and trypan blue exclusion. A total of 48 clinical isolates and 4 reference strains were included in the study (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiellaoxytoca, Acinetobacter baumannii, Serratia marcescens, Enterobacter cloacae). The results of the studies revealed that the MIC values differed between isolates, even within the same species. All the isolates were sensitive to TA-AgNPs in concentrations non-toxic to human cells during 24 h exposition. However, 48 h exposure to TA-AgNPs increased toxicity to HNEpC, narrowing their therapeutic window and enabling 19% of pathogens to resist the TA-AgNPs’ biocidal action. It was concluded that TA-AgNPs are non-toxic for the investigated eukaryotic cells after short-term exposure and effective against most pathogens isolated from patients with AECRS, but sensitivity testing may be necessary before application.
Funder
Jagiellonian University Medical College statutory funds
European Social Fund
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference71 articles.
1. European Position Paper on Rhinosinusitis and Nasal Polyps 2020;Fokkens;Rhinology,2020
2. International consensus statement on allergy and rhinology: Rhinosinusitis;Orlandi;Int. Forum. Allergy Rhinol.,2020
3. Bacteriology of chronic sinusitis and acute exacerbation of chronic sinusitis;Brook;Arch. Otolaryngol. Head Neck Surg.,2006
4. Antibacterial Activity of Silver Nanoparticles: Structural Effects;Tang;Adv. Healthc. Mater.,2018
5. Gibała, A., Żeliszewska, P., Gosiewski, T., Krawczyk, A., Duraczyńska, D., Szaleniec, J., Szaleniec, M., and Oćwieja, M. (2021). Antibacterial and Antifungal Properties of Silver Nanoparticles-Effect of a Surface-Stabilizing Agent. Biomolecules, 11.
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