A Putative NADPH Oxidase Gene in Unicellular Pathogenic Candida glabrata Is Required for Fungal ROS Production and Oxidative Stress Response
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Published:2023-12-27
Issue:1
Volume:10
Page:16
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ISSN:2309-608X
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Container-title:Journal of Fungi
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language:en
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Short-container-title:JoF
Author:
Lin Maoyi1, Huang Yao1, Orihara Kanami1ORCID, Chibana Hiroji2ORCID, Kajiwara Susumu1, Chen Xinyue1ORCID
Affiliation:
1. School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan 2. Medical Mycology Research Center, Chiba University, Chiba 263-8522, Japan
Abstract
Most previous studies on fungal NADPH oxidases (Nox) focused on multicellular fungi and highlighted the important roles of Nox-derived reactive oxygen species (ROS) in cellular differentiation and signaling communication. However, there are few reports about Nox in unicellular fungi. A novel NOX ortholog, CAGL0K05863g (named CgNOX1), in Candida glabrata was investigated in this study. Deletion of CgNOX1 led to a decrease in both intracellular and extracellular ROS production. In addition, the Cgnox1∆ mutant exhibited hypersensitivity to hydrogen peroxide and menadione. Also, the wild-type strain showed higher levels of both CgNOX1 mRNA expression and ROS production under oxidative stress. Moreover, the absence of CgNOX1 resulted in impaired ferric reductase activity. Although there was no effect on in vitro biofilm formation, the CgNOX1 mutant did not produce hepatic apoptosis, which might be mediated by fungal Nox-derived ROS during co-incubation. Together, these results indicated that the novel NOX gene plays important roles in unicellular pathogenic C. glabrata and its interaction with host cells.
Funder
JSPS KAKENHI JST SPRING
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
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