Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium among Patients with Urogenital Symptoms in Istanbul
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Published:2023-03-23
Issue:7
Volume:11
Page:930
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ISSN:2227-9032
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Container-title:Healthcare
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language:en
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Short-container-title:Healthcare
Author:
Kirkoyun Uysal Hayriye1ORCID, Koksal Muammer Osman1ORCID, Sarsar Kutay1ORCID, Ilktac Mehmet2, Isik Zeynep1, Akgun Karapinar Deniz Bahar1, Demirci Mehmet3ORCID, Ongen Betigul1, Buyukoren Ahmet4, Kadioglu Ates5, Yurtsever Eray6, Agacfidan Ali1
Affiliation:
1. Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey 2. Faculty of Pharmacy, Eastern Mediterranean University, Famagusta 99450, Cyprus 3. Department of Medical Microbiology, Faculty of Medicine, Kirklareli University, Kırklareli 39100, Turkey 4. Department of Obstetrics and Gynecology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey 5. Department of Urology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey 6. Department of Biostatistics, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey
Abstract
Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium are the three most commonly reported sexually transmitted bacteria. The present study aimed to investigate the presence of C. trachomatis, N. gonorrhoeae, and M. genitalium in urogenital samples collected from 18–68-year-old Turkish patients who were admitted to the hospital with various urogenital symptoms. A total of 360 patients with symptoms of STD were included in the study. Following DNA extraction by QIAamp Mini Kit, the presence of C. trachomatis, N. gonorrhoeae, and M. genitalium were investigated using multiplex real-time PCR. Causative organisms were identified in 68 (18.9%) of 360 patients. C. trachomatis, N. gonorrhoeae, and M. genitalium were detected in 40 (11.1%), 14 (3.9%), and 28 (7.8%) of the patients, respectively. Patients 21–30 years of age represented more than one-third (37.8%) of positive patients. Of all patients, dual infections of C. trachomatis–M. genitalium, N. gonorrhoeae–C. trachomatis, N. gonorrhoeae–M. genitalium, and triple infection of C. trachomatis–N. gonorrhoeae–M. genitalium were determined in 1.6% (6/360), 1.3% (5/360), 0.2% (1/360), and 0.2% (1/360) of the patients, respectively. In CT-, NG-, and MG-positive patients, different STI agents were also found such as HIV, HBV, HPV, HSV2, T. pallidum, and T. vaginalis. In conclusion, among C. trachomatis, N. gonorrhoeae, and M. genitalium, CT was the most frequently detected bacterial cause of STDs in our hospital at Istanbul. Co-infections, which comprise more than one-fifth of the cases, should not be underestimated. Regular screening and following up of STD agents using multiplex real-time PCR-based diagnostic methods enabling the immediate detection of co-infections are essential for the treatment and primary prevention of STDs.
Subject
Health Information Management,Health Informatics,Health Policy,Leadership and Management
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