Affiliation:
1. Departamento de Alimentos y Biotecnología, Facultad de Química, UNAM, Mexico City 04510, Mexico
Abstract
Poly(lactic-acid) (PLA) is a biodegradable polymer widely used as a packaging material. Its monomer, lactic acid, and its derivatives have been used in the food, cosmetic, and chemical industries. The accumulation of PLA residues leads to the development of green degrading methodologies, such as enzymatic degradation. This work evaluates the potential use of three cutinolytic enzymes codified in the Aspergillus nidulans genome to achieve this goal. The results are compared with those obtained with proteinase K from Tritirachium album, which has been reported as a PLA-hydrolyzing enzyme. The results show that all three cutinases act on the polymer, but ANCUT 1 releases the highest amount of lactic acid (25.86 mM). Different reaction conditions assayed later led to double the released lactic acid. A decrease in weight (45.96%) was also observed. The enzyme showed activity both on poly L lactic acid and on poly D lactic acid. Therefore, this cutinase offers the potential to rapidly degrade these package residues, and preliminary data show that this is feasible.
Reference36 articles.
1. Biopolymers potential biodegradable packaging material for food industry;Alavi;Polymers for Packaging Applications,2014
2. Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: How far are we?;Wei;Microb. Biotechnol.,2017
3. Kumar, V., Kumar, R., Singh, J., and Kumar, P. (2019). Contaminants in Agriculture and Environment: Health Risks and Remediation, Agro Environ Media, Publication Cell of AESA, Agriculture and Environmental Science Academy.
4. The development and challenges of poly (lactic acid) and poly (glycolic acid);Jem;Adv. Ind. Eng. Polym. Res.,2020
5. Sengupta, S., Manna, S., Roy, U., and Das, P. (2018). Manufacturing of Biodegradable Poly Lactic Acid (PLA): Green Alternatives to Petroleum Derived Plastics. Reference Module in Materials Science and Materials Engineering, Elsevier.