APHRODITE: A Compact Lab-on-Chip Biosensor for the Real-Time Analysis of Salivary Biomarkers in Space Missions

Author:

Nardi Lorenzo1ORCID,Davis Nithin Maipan1,Sansolini Serena1,Baratto de Albuquerque Thiago1,Laarraj Mohcine1,Caputo Domenico2ORCID,de Cesare Giampiero2ORCID,Shariati Pour Seyedeh Rojin3,Zangheri Martina3ORCID,Calabria Donato45ORCID,Guardigli Massimo45,Balsamo Michele6ORCID,Carrubba Elisa6,Carubia Fabrizio6,Ceccarelli Marco6,Ghiozzi Michele6,Popova Liyana6,Tenaglia Andrea6,Crisconio Marino7ORCID,Donati Alessandro6,Nascetti Augusto1ORCID,Mirasoli Mara35ORCID

Affiliation:

1. School of Aerospace Engineering, Sapienza University of Rome, Via Salaria 851, I-00138 Rome, Italy

2. Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Via Eudossiana 18, I-00184 Rome, Italy

3. Department of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, Tecnopolo di Rimini, Via Dario Campana 71, I-47922 Rimini, Italy

4. Department of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, Via Selmi 2, I-40126 Bologna, Italy

5. Interdepartmental Centre for Industrial Aerospace Research (CIRI AEROSPACE), Alma Mater Studiorum—University of Bologna, Via Baldassarre Canaccini 12, I-47121 Forlì, Italy

6. Kayser Italy S.r.l. Unipersonale, Via di Popogna 501, I-57128 Livorno, Italy

7. Agenzia Spaziale Italiana (ASI), Italian Space Agency, Via del Politecnico, I-00133 Rome, Italy

Abstract

One of the main challenges to be faced in deep space missions is to protect the health and ensure the maximum efficiency of the crew by preparing methods of prevention and in situ diagnosis. Indeed, the hostile environment causes important health problems, ranging from muscle atrophy, osteopenia, and immunological and metabolic alterations due to microgravity, to an increased risk of cancer caused by exposure to radiation. It is, therefore, necessary to provide new methods for the real-time measurement of biomarkers suitable for deepening our knowledge of the effects of space flight on the balance of the immune system and for allowing the monitoring of the astronaut’s health during long-term missions. APHRODITE will enable human space exploration because it fills this void that affects both missions in LEO and future missions to the Moon and Mars. Its scientific objectives are the design, production, testing, and in-orbit demonstration of a compact, reusable, and reconfigurable system for performing the real-time analysis of oral fluid samples in manned space missions. In the frame of this project, a crew member onboard the ISS will employ APHRODITE to measure the selected target analytes, cortisol, and dehydroepiandrosterone sulfate (DHEA-S), in oral fluid, in four (plus one additional desired session) separate experiment sessions. The paper addresses the design of the main subsystems of the analytical device and the preliminary results obtained during the first implementations of the device subsystems and testing measurements on Earth. In particular, the system design and the experiment data output of the lab-on-chip photosensors and of the front-end readout electronics are reported in detail along with preliminary chemical tests for the duplex competitive CL-immunoassay for the simultaneous detection of cortisol and DHEA-S. Different applications also on Earth are envisaged for the APHRODITE device, as it will be suitable for point-of-care testing applications (e.g., emergency medicine, bioterrorism, diagnostics in developing countries, etc.).

Funder

Italian Space Agency

Publisher

MDPI AG

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3