Affiliation:
1. Unit of Human Bacterial Diseases, Sciensano, 1050 Brussels, Belgium
2. Elanco Austria GmbH, Quartier Belvedere Central, 1100 Vienna, Austria
Abstract
The timely differentiation of the AviPro Salmonella VAC T and VAC E strains from the wild-type Salmonella enterica ser. Typhimurium and ser. Enteritidis isolates is crucial for effectively monitoring veterinary isolates. Currently, the distinction between field and vaccine strains has been conducted routinely via phenotypic antimicrobial resistance testing since the vaccines were first introduced more than 20 years ago, and the differentiation based on the antimicrobial resistance profile is still a valid and well-established method. However, an alternative method was sought for those laboratories that prefer a PCR-based method for logistic and/or operational reasons. In this study, we developed two triplex Real-Time PCR reactions that targeted conserved and specific mutations and, therefore, enabled the reliable differentiation of field and vaccine strains. To validate the effectiveness of both assays, we extensively tested them on a dataset consisting of 405 bacterial strains. The results demonstrated a 100% sensitivity and specificity for distinguishing both Salmonella enterica ser. Typhimurium and Enteritidis, although a confirmed culture is required.
Reference18 articles.
1. EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards) (2013). Scientific Opinion on the public health hazards to be covered by inspection of meat from farmed game. EFSA J., 11, 326.
2. Salmonella in eggs and egg-laying chickens: Pathways to effective control;Gast;Crit. Rev. Microbiol.,2022
3. European Commission (2003). Regulation
4. (EC) No 2160/2003 of the European Parliament and of the Council of 17 November 2003 on the control of Salmonella and other specified food-borne zoonotic agents. Off. J. Eur. Union, 46, 1-16.
5. Salmonella and Eggs: From Production to Plate;Whiley;Int. J. Environ. Res. Public Health,2015