Evidence of Nrf2/Keap1 Signaling Regulation by Mitochondria-Generated Reactive Oxygen Species in RGK1 Cells-Reference-Cited by-同舟云学术

Evidence of Nrf2/Keap1 Signaling Regulation by Mitochondria-Generated Reactive Oxygen Species in RGK1 Cells

Published:2023-02-27 Issue:3 Volume:13 Page:445
ISSN:2218-273X
Container-title:Biomolecules
language:en
Short-container-title:Biomolecules

Author:

Indo Hiroko¹²,Masuda Daisuke³⁴,Sriburee Sompong¹⁵,Ito Hiromu¹⁶,Nakanishi Ikuo⁶^ORCID,Matsumoto Ken-ichiro⁷^ORCID,Mankhetkorn Samlee⁵,Chatatikun Moragot⁸⁹^ORCID,Surinkaew Sirirat⁸¹⁰^ORCID,Udomwech Lunla¹¹,Kawakami Fumitaka¹²¹³¹⁴^ORCID,Ichikawa Takafumi¹²¹³,Matsui Hirofumi¹⁵^ORCID,Tangpong Jitbanjong⁸¹⁰,Majima Hideyuki¹²³⁸⁹¹⁰

Affiliation:

1. Department of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan

2. Amanogawa Galactic Astronomy Research Center (AGARC), Graduate School of Sciences, Kagoshima University, Kagoshima 890-0065, Japan

3. Department of Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan

4. Utilization & Engineer Department, Japan Manned Space Systems Corporation, 21-6 Tsukuba, Tsukuba 305-0047, Japan

5. Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand

6. Quantum RedOx Chemistry Team, Institute for Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Chiba 263-8555, Japan

7. Quantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, National Institute of Radiological Sciences, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology (QST), Chiba 263-8555, Japan

8. School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand

9. Center of Excellence Research for Melioidosis and Microorganisms, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand

10. Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand

11. School of Medicine, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand

12. Department of Regulation Biochemistry, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan

13. Department of Health Administration, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan

14. Regenerative Medicine and Cell Design Research Facility, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan

15. Division of Gastroenterology, Graduate School of Comprehensive Human Sciences, University Tsukuba, Tsukuba 305-8575, Japan

Abstract

It has been known that reactive oxygen species (ROS) are generated from the mitochondrial electron transport chain (ETC). Majima et al. proved that mitochondrial ROS (mtROS) caused apoptosis for the first time in 1998 (Majima et al. J Biol Chem, 1998). It is speculated that mtROS can move out of the mitochondria and initiate cellular signals in the nucleus. This paper aims to prove this phenomenon by assessing the change in the amount of manganese superoxide dismutase (MnSOD) by MnSOD transfection. Two cell lines of the same genetic background, of which generation of mtROS are different, i.e., the mtROS are more produced in RGK1, than in that of RGM1, were compared to analyze the cellular signals. The results of immunocytochemistry staining showed increase of Nrf2, Keap1, HO-1 and 2, MnSOD, GCL, GST, NQO1, GATA1, GATA3, GATA4, and GATA5 in RGK1 compared to those in RGM1. Transfection of human MnSOD in RGK1 cells showed a decrease of those signal proteins, suggesting mtROS play a role in cellular signals in nucleus.

Funder

JSPS (the Japan Society for the Promotion of Science) Core-to-Core Program

Publisher

MDPI AG

Subject

Molecular Biology,Biochemistry

Link

https://www.mdpi.com/2218-273X/13/3/445/pdf

Reference67 articles.

1. Mitochondria as source of free radicals;Naito;Free Radical Biology in Digestive Diseases,2010

2. Chasing great paths of Helmut Sies “Oxidative Stress”;Majima;Arch. Biochem. Biophys.,2016

3. On the origin of cancer cells;Warburg;Science,1956

4. Revisiting the Warburg effect: Historical dogma versus current understanding;Vaupel;J. Physiol.,2021

5. Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage;Indo;Mitochondrion,2007

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