Efficacy and Potential Mechanism of Essential Oils of Three Labiatae Plants against the Pathogenic Fungi of Root Rot Disease in Atractylodes chinensis
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Published:2023-10-15
Issue:10
Volume:9
Page:1136
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ISSN:2311-7524
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Container-title:Horticulturae
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language:en
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Short-container-title:Horticulturae
Author:
Xie Siyuan123, Si He123, Zhang Shenfei123, Zhou Ru123, Xue Yuyan123, Wang Shijie123, Wang Shiqiang123ORCID, Duan Yizhong4, Niu Junfeng123ORCID, Wang Zhezhi123ORCID
Affiliation:
1. National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China 2. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, The Ministry of Education, Xi’an 710119, China 3. College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China 4. College of Life Sciences, Yulin University, Yulin 718000, China
Abstract
Atractylodes chinensis has a high medicinal value and is widely cultivated. However, root rot disease seriously affects the yield and quality of A. chinensis. To develop green and safe pesticides, the inhibitory effect of essential oils (EOs) of three Labiatae plants on the pathogenic fungi that causes root rot disease in Atractylodes chinensis was investigated. The results showed that the Origanum vulgare EO and Thymus mongolicus EO exhibited strong inhibitory effects on Fusarium oxysporum, Fusarium solani, and Fusarium redolens, with 100% inhibition rate. The low MIC values of EOs and their main components against the three pathogenic fungi indicated that all of them showed strong fungicidal effects. The MIC values of O. vulgare EO against F. oxysporum, F. solani, and F. redolens were 2.60 mg/mL, 3.13 mg/mL, and 1.56 mg/mL, respectively. Analyses using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the O. vulgare EO severely damaged the cell wall and cell membrane of mycelial cells. The O. vulgare EO increased cell permeability, leading to a large leakage of cell contents (DNA and proteins). In addition, O. vulgare EO inhibited F. oxysporum by inducing ROS production and reducing the amount of intracellular GSH, leading to a large accumulation of ROS. This study showed that plant EOs have excellent fungicidal activity and can be used as novel natural and environmentally friendly pesticides for the control of root rot in A. chinensis.
Funder
Xi’an Science and Technology Project Fundamental Research Funds for the Central Universities Shaanxi Provincial Key R & D Program Youth Innovation Team Construction Scientific Research Project of Shaanxi Education Department Shaanxi Administration of Traditional Chinese Medicine Projects
Subject
Horticulture,Plant Science
Reference83 articles.
1. In vitro production of atractylon and β-eudesmol from Atractylodes chinensis by adventitious root culture;Yun;Appl. Microbiol. Biotechnol.,2022 2. Wang, L., Zhang, H., Wu, X., Wang, Z., Fang, W., Jiang, M., Chen, H., Huang, L., and Liu, C. (2020). Phylogenetic relationships of Atractylodes lancea, A. chinensis and A. macrocephala, revealed by complete plastome and nuclear gene sequences. PLoS ONE, 15. 3. HS-SPME coupled with GC-MS for elucidating differences between the volatile components in wild and cultivated Atractylodes chinensis;Lei;Phytochem. Anal.,2023 4. Zhao, J., Sun, C., Shi, F., Ma, S., Zheng, J., Du, X., and Zhang, L. (2021). Comparative transcriptome analysis reveals sesquiterpenoid biosynthesis among 1-, 2- and 3-year old Atractylodes chinensis. BMC Plant Biol., 21. 5. Ma, Z., Liu, G., Yang, Z., Zhang, G., Sun, L., Wang, M., and Ren, X. (2023). Species Differentiation and Quality Evaluation for Atractylodes Medicinal Plants by GC-MS coupled with Chemometric Analysis. Chem. Biodivers., 20.
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