The Effects of Foliar Salicylic Acid and Zinc Treatments on Proline, Carotenoid, and Chlorophyll Content and Anti-Oxidant Enzyme Activity in Galanthus elwesii Hook

Abstract

Galanthus elwesii Hook. is an important plant species of the Amaryllidaceae family and is used for the medicinal purposes of its valuable bioactive compounds. The present study was conducted to investigate the effects of foliar salicylic acid (SA) and zinc (Zn) treatments on the proline, carotenoid, and chlorophyll content and the anti-oxidant enzyme activity in G. elwesii. The ascorbate peroxidase (APX) enzyme activity, catalase (CAT) enzyme activity, and protein contents were determined with ascorbate oxidation, hydrogen peroxide (H2O2), and Bradford experiments, respectively. The plants were treated with three different concentrations of SA (0.5, 1, and 2 mM) and Zn (40, 80, and 120 mM) and were compared with the control. Fresh leaves were harvested in the study. APX (3.99 ± 0.58 EU/mg protein) and CAT (154.64 ± 4.10 EU/mg protein) were obtained from Zn 80 and 120 mM treatments at the highest level, respectively. The proline, chlorophyll b, and carotenoid content increased 12.4, 1.54, and 3.95-fold, respectively, in 0.5 mM SA treatments, when matched with the control group. It was found that increasing doses of SA and Zn increased the content of malondialdehyde (MDA), but this was not at a significant level. The total chlorophyll content increased 2.27-fold in Zn 120 mM + SA 2 mM treatment and the chlorophyll content increased 2.41-fold in Zn 40 mM + SA 1 mM treatment.