Affiliation:
1. State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Horticulture, Northwest A&F University, Xianyang 712100, China
2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Xianyang 712100, China
Abstract
SWEET (Sugars Will Eventually Be Exported Transporter) proteins, identified recently as a novel class of sugar transporters, play pivotal roles in the transport and distribution of photosynthetic products in plants. They are integral to physiological processes such as response to biotic and abiotic stress, growth and development, and fruit quality formation. In this study, leveraging the latest grapevine genomic data, we identified 18 members of the grapevine SWEET family and named them based on their homologs in Arabidopsis. We conducted a detailed analysis of these protein-encoding genes, focusing on their structure, conserved domains, and phylogenetic relationships. Phylogenetic analysis revealed that the grapevine SWEET family members could be categorized into four clades, with the majority of members displaying relatively conserved gene structures and motifs. Chromosomal localization and homology analysis indicated an uneven distribution of VvSWEETs across 11 chromosomes, with evidence of two segmental duplication events during evolution. Furthermore, we investigated the transcription levels of SWEET genes across different tissues, organs, and developmental stages of fruit, as well as their response patterns under abiotic stress (drought, cold, and salt stress) and biotic stress (Botrytis cinerea infection). Expression profiling demonstrated strong tissue-specificity and temporal-spatial specificity of VvSWEETs, correlated with their respective clades. It is noteworthy that the expression levels of most members within Clade 1 of the VvSWEET gene family, especially VvSWEET1, were markedly upregulated in response to a broad range of stress conditions. Our results provide a comprehensive bioinformatic characterization and analysis of the grapevine SWEET gene family, unveiling the potential functions of grapevine SWEET genes and offering a vital reference for further functional studies.
Funder
National Natural Science Foundation of China Grant
Natural Science Basic Research Program of Shaanxi
Reference86 articles.
1. Sugar compartmentation as an environmental stress adaptation strategy in plants;Yamada;Semin. Cell Dev. Biol.,2017
2. In Concert: Orchestrated Changes in Carbohydrate Homeostasis Are Critical for Plant Abiotic Stress Tolerance;Pommerrenig;Plant Cell Physiol.,2018
3. Transport mechanisms for organic forms of carbon and nitrogen between source and sink;Lalonde;Annu. Rev. Plant Biol.,2004
4. Diverse functional roles of monosaccharide transporters and their homologs in vascular plants: A physiological perspective;Slewinski;Mol. Plant,2011
5. Sucrose transporters of higher plants;Grof;Curr. Opin. Plant Biol.,2010