Identification of Laccase Genes in Grapevine and Their Roles in Response to Botrytis cinerea
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Published:2024-04-09
Issue:4
Volume:10
Page:376
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ISSN:2311-7524
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Container-title:Horticulturae
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language:en
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Short-container-title:Horticulturae
Author:
Wan Ran12ORCID, Yang Zhenfeng12, Liu Jun12, Zhang Mengxi12, Jiao Jian12, Wang Miaomiao12ORCID, Zhang Kunxi12ORCID, Hao Pengbo12, Liu Yu12, Bai Tuanhui12ORCID, Song Chunhui12, Song Shangwei12, Shi Jiangli12ORCID, Zheng Xianbo12
Affiliation:
1. College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China 2. Henan Key Laboratory of Fruit and Cucurbit Biology, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China
Abstract
Laccases are the key enzymes responsible for plant lignin biosynthesis and responses to environment stress. However, the roles of LAC genes in plant disease resistance are still largely unknown, especially in grapevine, one of the most important horticultural crops in the world. Its quality and yield are very vulnerable to gray mold disease caused by Botrytis cinerea. In total, 30 VvLAC genes were identified and found to be unevenly distributed on seven chromosomes; they were classified into seven groups based on phylogenetic analysis according to the criteria applied in Arabidopsis thaliana. Collinearity and synteny analyses identified some orthologous gene pairs in Vitis vinifera and a few paralogous gene pairs among grape and peach. The VvLAC gene family has diverse gene structures and a highly conserved motif composition. The prominent presence of the MYB cis-elements in each VvLAC promoter highlighted MYB transcriptional factors as the main regulators of VvLAC genes. Furthermore, twenty-five VvLAC genes with functional redundancy are probably implicated in grape lignin biosynthesis. The expression patterns of the LAC genes in grape leaves of Chinese wild V. amurensis ‘Shuangyou’ (SY), a germplasm highly resistant to B. cinerea, were investigated through transcriptomic data and qRT-PCR verification. Combined with the phylogenetic analysis, with AtLACs participating in lignin metabolism, and the cis-element analysis, VaLAC14, VaLAC19, VaLAC24 and VaLAC30 were identified as key candidate genes for lignin biosynthesis in the grape response to B. cinerea. This study supplies a comprehensive understanding of the classification, evolution, structure and responses of the grape LAC genes against B. cinerea. It also provides valuable genetic resources for functional characterization towards enhancing grapevine disease resistance.
Funder
Natural Science Foundation of Henan province National Natural Science Foundation of China
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