An Open-Circuit Indirect Calorimetry Head Hood System for Measuring Methane Emission and Energy Metabolism in Small Ruminants

Abstract

Methane (CH4) is a natural by-product of microbial fermentation in the rumen and is a powerful greenhouse gas. An open-circuit indirect calorimetry system for continuous determination of CH4 and CO2 production and O2 consumption and, thereafter, heat production (HP) calculation for small ruminants was described and validated. The system consisted of a computerized control, data acquisition and recording system for gases and air flux. The average value ± standard deviation for the calibration factors in the system were 1.005 ± 0.0007 (n = 6), 1.013 ± 0.0012 (n = 6) and 0.988 ± 0.0035 (n = 6) for O2, CO2 and CH4, respectively. Calibration factors close to 1 confirmed the absence of leaks in the indirect calorimetry system. In addition, an experimental test with 8 goats at mid lactation was conducted to validate the system. The repeatability for CH4 and heat production measured with the open-circuit indirect calorimetry system was 79% and 61%, respectively. Daily average HP measured by indirect calorimetry (Respiration Quotient method) was close to the average HP determined from Carbon-Nitrogen balance (CN method), accounting for 685 and 667 kJ per kg metabolic body weight, respectively. Therefore, discrepancies averaged 1.92%, a rather satisfactory value considering the substantial amount of technical and analytical work involved. The close agreement found between both methods can be considered as being indicative of the absence of systematic error. Two diets with different forage were tested: 40% was either alfalfa hay (HAY) or alfalfa silage (SIL), and the proportion of concentrate was the same in both groups (60%). The experimental trial shown that HP and CH4 were higher in HAY than SIL diet (differences between treatments of 28 kJ of HP per kg of metabolic body weight and 7.1 L CH4/day were found). The data acquisition and recording device developed improved the accuracy of the indirect calorimetry system by reducing the work involved in managing output data and refining the functionality for measuring gas exchange and energy metabolism in small ruminants.