Genome-Wide Identification and an Evolution Analysis of Tonoplast Monosaccharide Transporter (TMT) Genes in Seven Gramineae Crops and Their Expression Profiling in Rice
Author:
Zhou Mingao12ORCID, Deng Xiaoxiao3, Jiang Yifei12, Zhou Guoning4, Chen Jianmin1
Affiliation:
1. Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China 2. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China 3. The Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China 4. School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China
Abstract
The tonoplast monosaccharide transporter (TMT) family plays essential roles in sugar transport and plant growth. However, there is limited knowledge about the evolutionary dynamics of this important gene family in important Gramineae crops and putative function of rice TMT genes under external stresses. Here, the gene structural characteristics, chromosomal location, evolutionary relationship, and expression patterns of TMT genes were analyzed at a genome-wide scale. We identified six, three, six, six, four, six, and four TMT genes, respectively, in Brachypodium distachyon (Bd), Hordeum vulgare (Hv), Oryza rufipogon (Or), Oryza sativa ssp. japonica (Os), Sorghum bicolor (Sb), Setaria italica (Si), and Zea mays (Zm). All TMT proteins were divided into three clades based on the phylogenetic tree, gene structures, and protein motifs. The transcriptome data and qRT-PCR experiments suggested that each clade members had different expression patterns in various tissues and multiple reproductive tissues. In addition, the microarray datasets of rice indicated that different rice subspecies responded differently to the same intensity of salt or heat stress. The Fst value results indicated that the TMT gene family in rice was under different selection pressures in the process of rice subspecies differentiation and later selection breeding. Our findings pave the way for further insights into the evolutionary patterns of the TMT gene family in the important Gramineae crops and provide important references for characterizing the functions of rice TMT genes.
Funder
‘5511’ Collaborative Innovation project for High-quality Development and Surpasses of Agriculture between Government of Fujian Province and Chinese Academy of Agricultural Sciences
Subject
Genetics (clinical),Genetics
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