Current Approaches to and the Application of Intracytoplasmic Sperm Injection (ICSI) for Avian Genome Editing

Author:

Mizushima Shusei1,Sasanami Tomohiro2,Ono Tamao3,Kuroiwa Asato1

Affiliation:

1. Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo 060-0810, Japan

2. Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan

3. Matsumoto Dental University, 1780 Gobara, Hiro-oka, Shiojiri 399-0781, Nagano, Japan

Abstract

Poultry are one of the most valuable resources for human society. They are also recognized as a powerful experimental animal for basic research on embryogenesis. Demands for the supply of low-allergen eggs and bioreactors have increased with the development of programmable genome editing technology. The CRISPR/Cas9 system has recently been used to produce transgenic animals and various animals in the agricultural industry and has also been successfully adopted for the modification of chicken and quail genomes. In this review, we describe the successful establishment of genome-edited lines combined with germline chimera production systems mediated by primordial germ cells and by viral infection in poultry. The avian intracytoplasmic sperm injection (ICSI) system that we previously established and recent advances in ICSI for genome editing are also summarized.

Funder

Japan Society for the Promotion of Science

Nipponham Foundation for the Future of Food

Publisher

MDPI AG

Subject

Genetics (clinical),Genetics

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