Production and Antibacterial Activity of Atypical Siderophore from Pseudomonas sp. QCS59 Recovered from Harpachene schimperi

Abstract

Among sixty-eight pseudomonads, isolate QCS59 from the rhizosphere of H. schimperi was selected based on its siderophore level. Production was optimal in Kings B supplemented with 2% peptone and 0.5% fructose at pH 6.5 and 25 °C for 72 h. Additionally, the threshold potential of iron was found at a concentration of 10 µM. After purification, the acidified siderophore presented a maximum absorption peak of 360 nm, while the neutral form presented a maximum of 414 nm, confirming its pyoverdine (PVD) nature. Furthermore, a major peak appeared at a retention time (RT) of 27.5 min during RP-HPLC, confirming its homogeneity. Interestingly, it demonstrated effective antibacterial activity, especially against Escherichia coli ATCC 8739, with a minimum inhibitory concentration (MIC) of 6.3 µg/mL and a minimum bactericidal concentration (MBC) of 12.5 µg/mL. At ½ the MIC value, it inhibited 82.1% of well-established biofilms of Salmonella enterica. There was an increase in malondialdehyde (MDA) and antioxidative enzymes, especially catalase (CAT) in the treated bacteria because of the peroxidation of membrane lipids and oxidative stress, respectively. SEM proved cellular lysis and surface malformation in most of the treated bacteria. This study concludes that QCS59 siderophore is a promising antibacterial candidate for treating wastewater bacteria and skin pathogens.