Numerical-Sampling-Functionalized Real-Time Index Regulation for Direct k-Domain Calibration in Spectral Domain Optical Coherence Tomography

Abstract

An index-regulation technique functionalized by numerical sampling for direct calibration of the non-linear wavenumber (k)-domain to a linear domain in spectral domain optical coherence tomography (SD-OCT) is proposed. The objective of the developed method is to facilitate high-resolution identification of microstructures in biomedical imaging. Subjective optical alignments caused by nonlinear sampling of interferograms in the k-domain tend to hinder depth-dependent signal-to-noise ratios (SNR) and axial resolution in SD-OCT. Moreover, the optical-laser-dependent k-domain requires constant recalibrated in accordance with each laser transition, thereby necessitating either hardware or heavy software compensations. As the key feature of the proposed method, a relatively simple software-based k-domain mask calibration technique was developed to enable real-time linear sampling of k-domain interpolations whilst facilitating image observation through use of an index-regulation technique. Moreover, it has been confirmed that dispersion can be simultaneously compensated with noise residuals generated using the proposed technique, and that use of complex numerical or hardware techniques are no longer required. Observed results, such as fall-off, SNR, and axial resolution clearly exhibit the direct impact of the proposed technique, which could help investigators rapidly achieve optical-laser-independent high-quality SD-OCT images.