Affiliation:
1. Laboratoire Lumière Matière et Interfaces (LUMIN), UMR 9024, Ecole Normale Supérieure Paris Saclay, CentraleSupélec, CNRS, Université Paris-Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
Abstract
With the advent of many optofluidic and droplet microfluidic applications using laser-induced fluorescence (LIF), the need for a better understanding of the heating effect induced by pump laser excitation sources and good monitoring of temperature inside such confined microsystems started to emerge. We developed a broadband highly sensitive optofluidic detection system, which enabled us to show for the first time that Rhodamine-B dye molecules can exhibit standard photoluminescence as well as blue-shifted photoluminescence. We demonstrate that this phenomenon originates from the interaction between the pump laser beam and dye molecules when surrounded by the low thermal conductive fluorocarbon oil, generally used as a carrier medium in droplet microfluidics. We also show that when the temperature is increased, both Stokes and anti-Stokes fluorescence intensities remain practically constant until a temperature transition is reached, above which the fluorescence intensity starts to decrease linearly with a thermal sensitivity of about −0.4%/°C for Stokes emission or −0.2%/°C for anti-Stokes emission. For an excitation power of 3.5 mW, the temperature transition was found to be about 25 °C, whereas for a smaller excitation power (0.5 mW), the transition temperature was found to be about 36 °C.
Subject
Electrical and Electronic Engineering,Mechanical Engineering,Control and Systems Engineering
Reference31 articles.
1. Influence de la température sur la fluorescence et la loi de Stokes;Radium,1910
2. Efficiency of anti-stokes fluorescence in dyes;Jablonski;Nature,1933
3. Absorption processes associated with anti-stokes fluorescence in rhodamine B solutions;Jain;J. Appl. Phys.,1973
4. Anti-stokes fluorescence imaging of microscale thermal fields in thin films;Kuzmin;J. Appl. Phys.,2011
5. Application of a temperature-dependent fluorescent dye (Rhodamine B) to the measurement of radiofrequency radiation-induced temperature changes in biological samples;Chen;Bioelectromagn. J. Bioelectromagn. Soc. Soc. Phys. Regul. Biol. Med. Eur. Bioelectromagn. Assoc.,2009
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献