Experimentally Induced Hyperglycemia in Prepubertal Phase Impairs Oocyte Quality and Functionality in Adult Mice

Author:

Predheepan Dhakshanya1,Daddangadi Akshatha1,Uppangala Shubhashree2,Laxminarayana Sindhura Lakshmi Koulmane3,Raval Keyur45,Kalthur Guruprasad6ORCID,Kovačič Borut7,Adiga Satish Kumar1ORCID

Affiliation:

1. Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal 576104 , India

2. Division of Reproductive Genetics, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal 576104 , India

3. Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal 576104 , India

4. D , India

5. epartment of Chemical Engineering, National Institute of Technology Karnataka Surathkal 575025 , India

6. Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal 576104 , India

7. Laboratory of Reproductive Biology, Department of Reproductive Medicine and Endocrinology, University Medical Centre , Maribor 2000 , Slovenia

Abstract

Abstract Reproductive abnormalities in women with a history of childhood diabetes are believed to be partially attributed to hyperglycemia. Prolonged hyperglycemia can negatively affect ovarian function and fertility during reproductive life. To address this in an experimental setting, the present study used streptozotocin-induced hyperglycemic prepubertal mouse model. The impact of prolonged hyperglycemic exposure during prepubertal life on ovarian function, oocyte quality, and functional competence was assessed in adult mice. The ovarian reserve was not significantly altered; however, the in vitro maturation potential (P < 0.001), mitochondrial integrity (P < 0.01), and meiotic spindle assembly (P < 0.05-0.001) in oocytes were significantly affected in hyperglycemic animals in comparison to control groups. The results from the study suggest that prepubertal hyperglycemia can have adverse effects on the oocyte functional competence and spindle integrity during the reproductive phase of life. Because these changes can have a significant impact on the genetic integrity and developmental potential of the embryos and fetus, the observation warrants further research both in experimental and clinical settings.

Funder

Science and Engineering Research Board

Publisher

The Endocrine Society

Subject

Endocrinology

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