Experimental assessment of the protective effect of hydroxymethyluracil complex compounds under extreme exposure to acrylamide

Abstract

Introduction. Acrylamide is widely used in industry. Considering the potential danger of acute acrylamide poisoning, it seems urgent to search for new effective ways to protect and increase the body’s resistance to extreme exposure. The purpose of the study is to conduct a comparative assessment of the protective effect of hydroxymethyluracil complex compounds when administering moderate lethal doses of acrylamide to laboratory animals. Materials and methods. The subacute experiment was conducted on thirty outbred male rats weighted of 189–194 g. For 14 days, a toxicant was administered intragastrically at a dose of 20 mg per kg of body weight, then a single dose of 200 mg per kg of body weight was administered. Animals received complex compounds of hydroxymethyluracil with ascorbic acid and sodium succinate (50 mg per kg body weight), with acetylcysteine (500 mg per kg body weight) one hour before acrylamide. Against the background of the administration of complex compounds without acrylamide to the animals. Then one week later, an analysis of the mortality was carried out, and the weight coefficients of the liver and kidneys were determined in the surviving animals. Results. When acrylamide was administered to animals at the level of average lethal doses, 4 out of 6 animals died in the positive control group. In the groups in which animals received, in addition to acrylamide, the compound of hydroxymethyluracil with ascorbic acid and sodium succinate, the death rate was 2 out of 6 rats. Among the animals that received the compound of hydroxymethyluracil with acetylcysteine, no mortality was recorded within 7 days, as in the group negative control. Limitations of the study are that the protective effect of hydroxymethyluracil complex compounds was assessed only on one species and gender of laboratory animals. Conclusion. The complex compound of hydroxymethyluracil with acetylcysteine has a significant protective effect on male rats under extreme exposure to acrylamide at the level of moderate lethal doses. For a more complete understanding of the mechanism of action and a final judgment about the protective properties of the being studied complex compounds, it is necessary to continue research with analysis of changes in other indicators of the health status of laboratory animals.