Affiliation:
1. Department of Chemical Engineering University of California Santa Barbara Santa Barbara California USA
2. Bioengineering Program University of California Santa Barbara California USA
3. Joint BioEnergy Institute (JBEI) Emeryville California USA
Abstract
AbstractMembrane‐embedded transporters impart essential functions to cells as they mediate sensing and the uptake and extrusion of nutrients, waste products, and effector molecules. Promiscuous multidrug exporters are implicated in resistance to drugs and antibiotics and are highly relevant for microbial engineers who seek to enhance the tolerance of cell factory strains to hydrophobic bioproducts. Here, we report on the identification of small multidrug resistance (SMR) transporters in early‐branching anaerobic fungi (Neocallimastigomycetes). The SMR class of transporters is commonly found in bacteria but has not previously been reported in eukaryotes. In this study, we show that SMR transporters from anaerobic fungi can be produced heterologously in the model yeast Saccharomyces cerevisiae, demonstrating the potential of these proteins as targets for further characterization. The discovery of these novel anaerobic fungal SMR transporters offers a promising path forward to enhance bioproduction from engineered microbial strains.
Funder
Army Research Office
National Science Foundation
U.S. Department of Energy
National Institute of General Medical Sciences
Subject
Molecular Biology,Biochemistry
Cited by
1 articles.
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