Cause of and countermeasures for oxidation of the cysteine-derived reagent used in the amino acid derivative reactivity assay

Author:

Fujita Masaharu1ORCID,Yamamoto Yusuke1,Watanabe Shinichi2,Sugawara Tsunetsugu2,Wakabayashi Koji3,Tahara Yu3,Horie Nobuyuki4,Fujimoto Keiichi4,Kusakari Kei5,Kurokawa Yoshihiko5,Kawakami Tsuyoshi6,Kojima Kohichi7,Kojima Hajime8,Ono Atsushi9,Katsuoka Yasuhiro1,Tanabe Hideto10,Yokoyama Hiroshi10,Kasahara Toshihiko1

Affiliation:

1. Fujifilm Corporation, Safety Evaluation Centre; Kanagawa Japan

2. Lion Corporation, Human & Environmental Safety Evaluation Center; Kanagawa Japan

3. Mitsui Chemicals, Inc.; Chemical Safety Department; Chiba Japan

4. Sumitomo Chemical Co., Ltd.; Environmental Health Science Laboratory; Osaka Japan

5. Nissan Chemical Corporation, Biological Research Laboratories; Saitama Japan

6. National Institute of Health Sciences; Division of Environmental Chemistry; Kanagawa Japan

7. Food and Drug Safety Center; Hatano Research Institute; Kanagawa Japan

8. National Institute of Health Sciences; Biological Safety Research Center, Division of Risk Assessment; Kanagawa Japan

9. Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences; Division of Pharmaceutical Sciences; Okayama Japan

10. Fujifilm Corporation, Research & Development Management Headquarters; Analysis Technology Center; Kanagawa Japan

Funder

Japan Chemical Industry Association

The Japanese Society for Alternatives to Animal Experiments

Publisher

Wiley

Subject

Toxicology

Reference42 articles.

1. Reactivity profiling: Covalent modification of single nucleophile peptides for skin sensitization risk assessment;Aleksic;Toxicological Sciences,2009

2. Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: Applications to soluble and transmembrane receptors of bacterial chemotaxis;Bass;Methods in Enzymology,2007

3. Casati , S. Aschberger , K. Asturiol , D. Basketter , D. Dimitrov , S. Dumont , C. Worth , A. 2016 Ability of non-animal methods for skin sensitisation to detect pre- and pro-haptens-Report and Recommendations of an EURL ECVAM Expert Meeting https://eurl-ecvam.jrc.ec.europa.eu/eurl-ecvam-status-reports/pre-prohapten-workshop-report

4. Oxidative protein folding in bacteria;Collet;Molecular Microbiology,2002

5. Oxidant sensing by reversible disulfide bond formation;Cremers;Journal of Biological Chemistry,2013

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