Affiliation:
1. Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in Wuhan Wuhan National Laboratory for Optoelectronics Wuhan Institute of Physics and Mathematics Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences 430071 Wuhan China
Abstract
AbstractIn vitro, 19F NMR methodology is preferably selected as a complementary and straightforward method for unveiling the conformations, dynamics, and interactions of biological molecules. Its effectiveness in vivo has seen continuous improvement, addressing challenges faced by conventional heteronuclear NMR experiments on structured proteins, such as severe line broadening, low signal‐to‐noise ratio, and background signals. Herein, we summarize the distinctive advantages of 19F NMR, along with recent progress in sample preparation and applications within the realm of in‐cell NMR. Additionally, we offer insights into the future directions and prospects of this methodology based on our understanding.
Funder
National Natural Science Foundation of China
Key Technologies Research and Development Program of Anhui Province
Cited by
3 articles.
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