Synthesis and characterization of N‐rich fluorescent bio‐dots as a reporter in the design of dual‐labeled FRET probe for TaqMan PCR: A feasibility study-Reference-Cited by-同舟云学术

Synthesis and characterization of N‐rich fluorescent bio‐dots as a reporter in the design of dual‐labeled FRET probe for TaqMan PCR: A feasibility study

Published:2022-08-17 Issue:2 Volume:70 Page:645-658
ISSN:0885-4513
Container-title:Biotechnology and Applied Biochemistry
language:en
Short-container-title:Biotech and App Biochem

Author:

Imani Mahsa¹,Mohajeri Nasrin¹,Rastegar Mojgan²,Zarghami Nosratollah¹³⁴^ORCID

Affiliation:

1. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences Tabriz Iran

2. Department of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences University of Manitoba Winnipeg Canada

3. Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran

4. Department of Medical Biochemistry, Faculty of Medicine Istanbul Aydin University Istanbul Turkey

Funder

Tabriz University of Medical Sciences

Publisher

Wiley

Subject

Process Chemistry and Technology,Drug Discovery,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,General Medicine,Bioengineering,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/bab.2387

Reference61 articles.

1. A beginner’s guide to RT-PCR, qPCR and RT-qPCR

2. Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore

3. Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control

4. Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay

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