Affiliation:
1. Maastricht University MERLN Institute for Technology‐Inspired Regenerative Medicine Complex Tissue Regeneration Department Maastricht 6229 ER The Netherlands
2. Institute of Pathology RWTH University of Aachen 52074 Aachen Germany
3. Division of Nephrology RWTH University of Aachen 52074 Aachen Germany
4. Electron Microscopy Facility RWTH University of Aachen 52074 Aachen Germany
Abstract
AbstractChronic kidney disease (CKD) ranks as the twelfth leading cause of death worldwide with limited treatment options. The development of in vitro models replicating defined segments of the kidney functional units, the nephrons, in a physiologically relevant and reproducible manner can facilitate drug testing. The aim of this study was to produce an in vitro organ‐on‐a‐chip platform with extrusion‐based three‐dimensional (3D) printing. The manufacturing of the tubular platform was produced by printing sacrificial fibers with varying diameters, providing a suitable structure for cell adhesion and proliferation. The chip platform was seeded with primary murine tubular epithelial cells and human umbilical vein endothelial cells. The effect of channel geometry, its reproducibility, coatings for cell adhesion, and specific cell markers were investigated. The developed chip presents single and dual channels, mimicking segments of a renal tubule and the capillary network, together with an extracellular matrix gel analogue placed in the middle of the two channels, envisioning the renal tubulointerstitium in vitro. The 3D printed platform enables perfusable circular cross‐section channels with fully automated, rapid, and reproducible manufacturing processes at low costs. This kidney tubulointerstitium on‐a‐chip provides the first step toward the production of more complex in vitro models for drug testing.
Funder
Nierstichting
Deutsche Forschungsgemeinschaft
H2020 Marie Skłodowska-Curie Actions
Subject
Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering,Biotechnology
Cited by
1 articles.
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