Bottom‐Up Extrusion‐Based Biofabrication of the Osteoid Niche

Author:

Parmentier Laurens1ORCID,D'Haese Sophie1,Carpentier Nathan1ORCID,Dmitriev Ruslan I.2ORCID,Van Vlierberghe Sandra1ORCID

Affiliation:

1. Polymer Chemistry and Biomaterials group (PBM) Centre of Macromolecular Chemistry (CMaC) Department of Organic and Macromolecular Chemistry Faculty of Sciences Ghent University Krijgslaan 281, Building S4‐Bis Ghent 9000 Belgium

2. Tissue Engineering and Biomaterials Group Department of Human Structure and Repair Faculty of Medical and Health Sciences Ghent university C. Heymanslaan 10 Ghent 9000 Belgium

Abstract

AbstractBone regeneration remains a clinical challenge given the transplantation incidence rate and the associated economic burden. Bottom‐up osteoid tissue engineering has the potential to offer an alternative approach to current clinical solutions that suffer from various drawbacks. In this paper, deposition‐based bioprinting is exploited while the effect is explored of both the crosslinking mechanism (gelatin methacryloyl (GelMA) versus gelatin norbornene (DS 91) crosslinked with thiolated gelatin (GelNBSH)) and the degree of substitution (GelNBSH versus norbornene‐norbornene‐modified gelatin (DS 169) crosslinked with thiolated gelatin (GelNBNBSH)) on the presented biophysical cues as well as on the osteogenic differentiation. The incorporation of tris(2‐carboxyethyl)phosphine (TCEP) to the step‐growth inks allows the production of reproducible and biocompatible scaffolds based on thiol‐ene chemistry. Dental pulp stem cell encapsulation in GelNBNBSH biofabricated constructs shows a favorable response due to the combination of its stress relaxation and substrate rigidity (bulk compressive modulus of 11–30 kPa) as reflected by a sevenfold increase in calcium production compared to the tissue engineering standard GelMA. This work is the first to exploit a controlled biocompatible and cell‐interactive thiolated macromolecular crosslinker (GelSH + TCEP) allowing the extrusion‐based biofabrication of low concentration (5 w/v%) modified osteogenic gelatin‐based inks (GelNBNBSH + TCEP).

Funder

Fonds Wetenschappelijk Onderzoek

Publisher

Wiley

Subject

Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3