Proper experimental design requires randomization/balancing of molecular ecology experiments

Author:

Bálint Miklós1ORCID,Márton Orsolya12,Schatz Marlene3,Düring Rolf-Alexander3,Grossart Hans-Peter45ORCID

Affiliation:

1. Senckenberg Biodiversity and Climate Research Centre; Frankfurt am Main Germany

2. Institute for Soil Sciences and Agricultural Chemistry; Centre for Agricultural Research; Hungarian Academy of Sciences; Budapest Hungary

3. Institut für Bodenkunde und Bodenerhaltung; Gießen Germany

4. Leibniz Institute for Freshwater Ecology and Inland Fisheries; Stechlin Germany

5. Institute of Biochemistry and Biology; Potsdam University; Potsdam Germany

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Nature and Landscape Conservation,Ecology,Ecology, Evolution, Behavior and Systematics

Reference36 articles.

1. Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries;Aird;Genome Biology,2011

2. Millions of reads, thousands of taxa: Microbial community structure and associations analyzed via marker genes;Bálint;FEMS Microbiology Reviews,2016

3. Massive influence of DNA isolation and library preparation approaches on palaeogenomic sequencing data;Barlow;BioRxiv,2016

4. Fitting linear mixed-effects models using lme4;Bates;Journal of Statistical Software,2015

5. obitools: A unix-inspired software package for DNA metabarcoding;Boyer;Molecular Ecology Resources,2015

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