Affiliation:
1. University of Miami Miller School of Medicine Miami Florida USA
2. College of Arts and Sciences University of Miami Coral Gables Florida USA
3. Department of Obstetrics, Gynecology and Reproductive Sciences University of Miami Miller School of Medicine Miami Florida USA
4. Department of Internal Medicine Larkin Community Hospital Miami Florida USA
5. Department of Biochemistry and Molecular Biology University of Miami Miller School of Medicine Miami Florida USA
Abstract
AbstractCOVID‐19, caused by SARS‐CoV‐2, has had a significant global impact. While vaccines and treatments have reduced severe cases and deaths, the long‐term effects are not yet well understood. Current models used for research, such as non‐human primates and transgenic mice, are expensive and require scarce Biosafety Level‐3 (BSL‐3) laboratories, thereby limiting their practicality. However, the mouse hepatitis virus 1 (MHV‐1) mouse model offers a promising alternative. This surrogate model can be investigated in more widely available Biosafety Level‐2 (BSL‐2) laboratories. Furthermore, mice are affordable and easy to handle, and utilizing MHV‐1 as a surrogate for SARS‐CoV‐2 eliminates the need for costly transgenic mice. Importantly, the MHV‐1 model successfully recapitulates COVID‐19‐related clinical symptoms, weight loss, multiorgan pathological changes and failure in acute stages, irreversible neurological complications, and other long‐term organ dysfunction post‐infection, which are similar to available human data post‐COVID‐19. To assist researchers in establishing and using the MHV‐1 mouse model, this protocol offers comprehensive guidance encompassing procedures for animal preparation, induction of viral infection, clinical observation, pathological changes, and tissue analysis for mechanistic studies, thereby yielding valuable insights into disease mechanisms and progression. By adopting the MHV‐1 model and the provided protocols, researchers can effectively circumvent financial constraints and the limited availability of BSL‐3 laboratories, thus facilitating a more accessible and cost‐effective approach to investigating the underlying mechanisms of SARS‐CoV‐2 pathophysiology and exploring potential therapeutic interventions. © 2023 Wiley Periodicals LLC.Basic Protocol: Induction of mouse hepatitis virus 1 (MHV‐1) infection in A/J miceSupport Protocol 1: Histological evaluationSupport Protocol 2: Liver enzyme measurementSupport Protocol 3: Western blot analysis of aquaporin expressionSupport Protocol 4: mRNA measurementSupport Protocol 5: Immunohistochemistry/immunofluorescenceSupport Protocol 6: Tissue water measurement
Subject
Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Cited by
3 articles.
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