Large‐scale separation of alkaloids from Corydalis decumbens by pH‐zone‐refining centrifugal partition chromatography and their anticomplement activity

Author:

Xie Xin‐Xin12,Chen Zhong‐Jian12,Zhu Quan‐Gang12,Yu Qin12,Lian Tian‐Yan12,Xu Xue‐Lian12,Chen Ya12,Song Wei‐Hua3ORCID

Affiliation:

1. Department of Pharmacy, Shanghai Skin Disease Hospital, School of Medicine Tongji University Shanghai P. R. China

2. Shanghai Engineering Research Center of External Chinese Medicine Shanghai P. R. China

3. Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine Shanghai Jiao Tong University Shanghai P. R. China

Abstract

Centrifugal partition chromatography in the pH‐zone‐refining mode was successfully applied to the separation of alkaloids from the crude extract of Corydalis decumbens. The experiment was performed with a two‐phase solvent system composed of petroleum ether‐ethyl acetate‐ethanol‐water (5:5:3:7, v/v/v/v) where triethylamine (10 mM) was added to the stationary phase and hydrochloric acid (10 mM) to the mobile phase. From 1.6 g of the crude extract, 43 mg protopine, 189 mg (+)‐egenine, and 158 mg tetrahydropalmatine were obtained with a purity of 98.2%, 94.6%, and 96.7%, respectively. Tetrahydropalmatine showed an interesting anticomplement effect with CH50 0.11 and AP50 0.25 mg/mL, respectively. In a mechanistic study, tetrahydropalmatine interacted with C1, C3, C4, and C5 components in the complement activation cascade.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Wiley

Subject

Filtration and Separation,Analytical Chemistry

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