Affiliation:
1. Department of Chemistry, Faculty of Science Kyushu University Fukuoka Japan
2. Department of Material Chemistry, Graduate School of Engineering Kyoto University Kyoto Japan
3. Research Administration Center Osaka Metropolitan University Osaka Japan
4. RIKEN Center for Biosystems Dynamics Research Osaka Japan
Abstract
The glycomic analysis holds significant appeal due to the diverse roles that glycans and glycoconjugates play, acting as modulators and mediators in cellular interactions, cell/organism structure, drugs, energy sources, glyconanomaterials, and more. The glycomic analysis relies on liquid‐phase separation technologies for molecular purification, separation, and identification. As a miniaturized form of liquid‐phase separation technology, microscale separation technologies offer various advantages such as environmental friendliness, high resolution, sensitivity, fast speed, and integration capabilities. For glycan analysis, microscale separation technologies are continuously evolving to address the increasing challenges in their unique manners. This review discusses the fundamentals and applications of microscale separation technologies for glycomic analysis. It covers liquid‐phase separation technologies operating at scales generally less than 100 µm, including capillary electrophoresis, nanoflow liquid chromatography, and microchip electrophoresis. We will provide a brief overview of glycomic analysis and describe new strategies in microscale separation and their applications in glycan analysis from 2014 to 2023.
Funder
Japan Society for the Promotion of Science
Japan Agency for Medical Research and Development
JST-Mirai Program