A CRISPR/Cas9‐engineered mouse carrying a conditional knockout allele for the early growth response‐1 transcription factor

Author:

Maurya Vineet K.1ORCID,Ying Yan1ORCID,Lanza Denise G.2ORCID,Heaney Jason D.2ORCID,Lydon John P.1ORCID

Affiliation:

1. Department of Molecular and Cellular Biology Baylor College of Medicine One Baylor Plaza Houston Texas 77030 USA

2. Department of Molecular and Human Genetics Baylor College of Medicine One Baylor Plaza Houston Texas 77030 USA

Abstract

SummaryEarly growth response 1 (EGR1) mediates transcriptional programs that are indispensable for cell division, differentiation, and apoptosis in numerous physiologies and pathophysiologies. Whole‐body EGR1 knockouts in mice (Egr1KO) have advanced our understanding of EGR1 function in an in vivo context. To extend the utility of the mouse to investigate EGR1 responses in a tissue‐ and/or cell‐type‐specific manner, we generated a mouse model in which exon 2 of the mouse Egr1 gene is floxed by CRISPR/Cas9 engineering. The floxed Egr1 alleles (Egr1f/f) are designed to enable spatiotemporal control of Cre‐mediated EGR1 ablation in the mouse. To confirm that the Egr1f/f alleles can be abrogated using a Cre driver, we crossed the Egr1f/f mouse with a global Cre driver to generate the Egr1 conditional knockout (Egr1d/d) mouse in which EGR1 expression is ablated in all tissues. Genetic and protein analysis confirmed the absence of exon 2 and loss of EGR1 expression in the Egr1d/d mouse, respectively. Moreover, the Egr1d/d female exhibits overt reproductive phenotypes previously reported for the Egr1KO mouse. Therefore, studies described in this short technical report underscore the potential utility of the murine Egr1 floxed allele to further resolve EGR1 function at a tissue‐ and/or cell‐type‐specific level.

Funder

National Institute of Child Health and Human Development

National Institutes of Health

Publisher

Wiley

Subject

Cell Biology,Endocrinology,Genetics

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