Selective and Sensitive Detection of Cu+ in Living Cells Based on Chelator Modified Carbon Dots

Author:

Gao Tian1ORCID,Deng Peixuan1ORCID,Guo Shengnan1ORCID,Zhang Jiaojiao1ORCID,Cai Yan1,Hu Zhicong1,Cai Qun1,Liu Yi123ORCID

Affiliation:

1. Interdisciplinary Institute of NMR and Molecular Sciences Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials School of Chemistry and Chemical Engineering Institute of Advanced Materials and Nanotechnology Wuhan University of Science and Technology Wuhan 430081 P. R. China

2. School of Chemical and Environmental Engineering Wuhan Polytechnic University Wuhan 430023 P. R. China

3. Hubei Key Laboratory of Radiation Chemistry and Functional Materials Hubei University of Science and Technology Xianning 437100 P. R. China

Abstract

AbstractCuprous ions (Cu+) are essential for various biological activities. However, it is still a great challenge to detect Cu+ efficiently in cells in situ. As emerging 0‐D carbon photoluminescence nanomaterials, carbon dots (CDs) exhibit non‐toxicity and ultra stability, which shows great advantages in biosensing and bioimaging. In this work, CDs are prepared by a one‐step microwave‐assisted hydrothermal method. To enhance the selectivity and efficiency of the CDs‐based sensor, the NS4‐CDs are synthesized by surface modification of CDs using Cu+ chelating molecules. The NS4‐CDs, that carry specific detection molecules, exhibit remarkable selectivity and high sensitivity with an extraordinarily low detection limit (26 nmol L−1). It is noteworthy that the detection response is extremely fast, which only takes 20 s. The PL response mechanism of NS4‐CDs is also thoroughly investigated. In addition, the NS4‐CDs possess great biocompatibility and non‐cytotoxicity, which is vital for advanced cellular imaging applications. Finally, the NS4‐CDs are successfully applied to Cu+ detection in cells in situ. This work paves a new path for the design and efficient construction of CDs‐based biosensors for ion detection in cells, which expands the application area of carbon nanomaterials.

Publisher

Wiley

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