A SNP‐based genotyping strategy to detect the abuse of homologous blood transfusion from dried blood spots-Reference-Cited by-同舟云学术

A SNP‐based genotyping strategy to detect the abuse of homologous blood transfusion from dried blood spots

Published:2024-01-31 Issue: Volume: Page:
ISSN:1942-7603
Container-title:Drug Testing and Analysis
language:en
Short-container-title:Drug Testing and Analysis

Author:

Donati Francesco¹,Natalucci Alice¹,Concetti Livia¹,de la Torre Xavier¹^ORCID,Botrè Francesco¹²^ORCID

Affiliation:

1. Laboratorio Antidoping Federazione Medico Sportiva Italiana Rome Italy

2. REDs—Research and Expertise in anti‐Doping sciences, ISSUL—Institute of Sport Sciences University of Lausanne Lausanne Switzerland

Abstract

AbstractWe performed genotyping analysis of human biallelic polymorphisms (single nucleotide polymorphisms) for the detection of homologous blood transfusion in sports doping. DNA was extracted from dried blood spots and quantified real‐time fast PCR. The method was proven to allow the detection of transfusions up to a donor percentage of 1%, with a significant improvement in terms of sensitivity with respect to both the reference cytofluorimetric method and a previously proposed strategy based on the DNA STR‐based strategy.

Publisher

Wiley

Reference18 articles.

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2. Blood doping in athletes-Detection of allogeneic blood transfusions by flow cytofluorometry

3. Scientific and forensic standards for homologous blood transfusion anti-doping analyses

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