Active, soluble recombinant melittin purified by extracting insoluble lysate ofEscherichia coliwithout denaturation

Author:

Buhrman Jason S.1,Rayahin Jamie E.1,Cook Laura C.2,Federle Michael J.2,Gemeinhart Richard A.134

Affiliation:

1. Dept. of Biopharmaceutical Sciences; University of Illinois; Chicago IL 60612-7231

2. Dept. of Medicinal Chemistry and Pharmacognosy; Center for Pharmaceutical Biotechnology, University of Illinois; Chicago IL 60607-7173

3. Dept. of Bioengineering; University of Illinois; Chicago IL 60607-7052

4. Dept. of Ophthalmology and Visual Sciences; University of Illinois; Chicago IL 60612-4319

Publisher

Wiley

Subject

Biotechnology

Reference29 articles.

1. Bee and wasp venoms;Habermann;Science.,1972

2. Mechanism of membrane damage induced by the amphipathic peptides gramicidin S and melittin;Katsu;Biochim Biophys Acta.,1989

3. A matrix metalloproteinase 2 cleavable melittin/avidin conjugate specifically targets tumor cells in vitro and in vivo;Holle;Int J Oncol.,2003

4. The water-soluble fraction of bee venom produces antinociceptive and anti-inflammatory effects on rheumatoid arthritis in rats;Kwon;Life Sci.,2002

5. On the mechanism of pore formation by melittin;van den Bogaart;J Biol Chem.,2008

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