Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC Pig‐a Workgroup

Author:

Dertinger Stephen D.1,Bhalli Javed A.2,Roberts Daniel J.3,Stankowski Leon F.3,Gollapudi B. Bhaskar4,Lovell David P.5,Recio Leslie6,Kimoto Takafumi7,Miura Daishiro7,Heflich Robert H.8

Affiliation:

1. Litron Laboratories Rochester New York USA

2. Covance Laboratories Inc. Greenfield Indiana USA

3. Charles River Laboratories Skokie Illinois USA

4. Toxicology Consulting Midland Michigan USA

5. St. George's University of London London UK

6. Integrated Laboratory Systems Research Triangle Park North Carolina USA

7. Teijin Pharma Limited Hino Tokyo Japan

8. U.S. Food and Drug Administration/NCTR Jefferson Arkansas USA

Publisher

Wiley

Subject

Health, Toxicology and Mutagenesis,Genetics(clinical),Epidemiology

Reference38 articles.

1. Suitability of Long-Term Frozen Rat Blood Samples for the Interrogation of Pig-a Gene Mutation by Flow Cytometry

2. Erythrocyte-Based Pig-a Gene Mutation Assay

3. Statistical Analysis of Monotone or Non-monotone Dose–Response Data from In Vitro Toxicological Assays

4. Standard protocol for the total red blood cell Pig-a assay used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen Society

5. Standard protocol for the PIGRET assay, a high‐throughput reticulocyte Pig‐a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society;Chikura S.;Genes and Environment,2021

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