Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Author:

Güler Muhammet Akif1ORCID,Çetin Bülent2ORCID,Albayrak Bülent3ORCID,Meral‐Aktaş Hacer2ORCID,Tekgündüz Kadir Şerafettin4ORCID,Kara Mustafa4ORCID,Işlek Ali5ORCID

Affiliation:

1. Division of Pediatric Nephrology, Department of Pediatrics Atatürk University, Faculty of Medicine Erzurum Türkiye

2. Department of Food Engineering, Faculty of Agriculture Ataturk University Erzurum Türkiye

3. Department of Gastroenterology, Faculty of Medicine Atatürk University Erzurum Türkiye

4. Division of Neonatology, Department of Pediatrics, Faculty of Medicine Atatürk University Erzurum Türkiye

5. Department of Pediatric Gastroenterology, Faculty of Medicine Çukurova University Adana Türkiye

Abstract

AbstractBACKGROUNDNeonatal feces are one of the most important sources for probiotic isolation. The purpose of this study was the isolation and identification of Bifidobacterium spp. from neonatal feces and the evaluation of in vitro probiotic properties of strains including safety tests.RESULTSA total of 40 isolates were obtained from 14 healthy newborns' feces in Erzurum province, Türkiye. By their rep‐PCR patterns and 16S rRNA gene sequences, isolates were identified as 26 Bifidobacterium breve and 14 Bifidobacterium longum. Fifteen of the isolates tolerated bile salts and showed high resistance to simulated gastric juice. Isolates exhibited varying rates of auto‐aggregation and hydrophobicity. In addition, most of the isolates displayed antibacterial activity against Escherichia coli O157:H7, Staphylococcus aureus ATCC 29213, Salmonella Typhimurium RSHMB 95091, and Pseudomonas aeruginosa ATCC 9027. However, only one strain showed bile salt hydrolase activity and two strains showed the ability to produce H2O2. Bifidobacterium strains were generally sensitive to the tested antibiotics and lacked kanamycin, gentamicin, and streptomycin resistance genes, and hemolytic and DNAse activities. On the other hand, it was determined that five strains had various virulence genes including gelE, esp, efaAfs, hyl, and ace.CONCLUSIONResults of the present study suggested that B. longum BH28, B. breve BH4 and B. breve BH5 strains have the potential as probiotic candidates for further studies. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Publisher

Wiley

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