Affiliation:
1. Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun, Jilin 130022 China
2. School of Applied Chemistry and Engineering University of Science and Technology of China Hefei, Anhui 230026 China
3. Department of Laboratory Medicine State Key Laboratory of Biotherapy and Cancer Center Clinica Laboratory Medicine Research Center West China Hospital Sichuan University Chengdu 610041 China
4. Tianfu Jincheng Laboratory City of Future Medicine Chengdu 610500 China
Abstract
AbstractPolystyrene (PS), a polymer material renowned for its notable optical and mechanical properties, as well as low cost and ease of processing, stands as one of optimal candidate for flexible electrode substrate materials. However, prevalent modification techniques such as lithography, electron beam evaporation, and plasma etching all entail the requirement for specialized equipment and precise operation, thereby significantly elevating the technical barrier. In this study, we introduce a chemical plating method to fabricate a gold film electrode, coupled with prussian blue (PB) and glucose oxidase (GOx), for the development of a glucose electrochemical biosensor. This process offers simplicity, cost‐effectiveness, and feasibility under regular experimental conditions. The electrode surface is covered with numerous gold nanoparticles, effectively augmenting the actual electrode area and enhancing electron transfer efficiency. Remarkably, without the addition of sensitizing agents, the biosensor achieves highly sensitive glucose detection. The proposed biosensor exhibits a broad linear relationship in the concentration range of 0.005–1 mM, with a detection limit of 4.4 μM. Moreover, it exhibits favorable performance in detecting glucose levels in sweat samples, showcasing promising prospects for personal healthcare diagnostics.