Facile preparation of a conjugated polymer‐based biosensor for glucose monitoring

Abstract

AbstractDiabetes is an important public health problem all over the world that affects the quality of life, and its late diagnosis leads to serious complications such as stroke, blindness, limb amputations, and kidney failure. Fabrication of economical and user‐friendly biosensor systems allows the rapid and early detection of prediabetes and type 2 diabetes. Accordingly, a novel, amino‐functionalized, conjugated, alternating polymer was synthesized, characterized, and coated on a glassy carbon electrode (GCE) to be used as an immobilization platform for the glucose oxidase (GOx) enzyme. The decline in oxygen level due to the enzymatic reaction at a potential of −0.7 V vs. silver/silver chloride (Ag/AgCl) is screened as a biosensor response. The proposed biosensor displayed high selectivity and specificity for glucose with a broad linear range, a low detection limit, good reproducibility and repeatability, and a long shelf life. Surface modifications were characterized using cyclic voltammetry (CV) and field emission scanning electron microscopy (FE‐SEM) techniques. The real‐life sample application of the constructed biosensor to determine the glucose amount was performed in human serum.