Near‐infrared light does not induce DNA damage in human dermal fibroblasts

Abstract

AbstractPhotobiomodulation (PBM) can be used to treat a range of conditions in dermatology. PBM refers to the changes induced by red (RL, 620–700 nm) and near‐infrared (NIR, 700–1440 nm) light. Light radiation‐induced DNA damage is a major contributor to aging and skin cancer. It is crucial to study the effects of PBM on DNA to ensure safety. Our lab previously demonstrated that RL (633 ± 6 nm) did not result in human dermal fibroblasts (HDFs) DNA damage. This study employed similar methods to investigate NIR effects. Commercially available LED‐NIR (830 ± 5 nm) panels (66, 132, and 264 J/cm2) did not result in DNA damage measured by cyclobutane pyrimidine dimers and pyrimidine‐6,4‐pyrimidone photoproducts in HDFs compared to temperature‐matched controls immediately, 3 h, and 24 h following irradiation and compared to positive and negative controls. This demonstrates that LED‐NIR does not damage DNA in HDFs in vitro.