Structure-based engineering of streptavidin monomer with a reduced biotin dissociation rate
Author:
Affiliation:
1. Department of Chemical and Biological Engineering; University at Buffalo; Buffalo New York 14260
2. Hauptman Woodward Institute; Buffalo New York 14203
3. Department of Structural Biology; University at Buffalo; Buffalo New York 14203
Publisher
Wiley
Subject
Molecular Biology,Biochemistry,Structural Biology
Reference44 articles.
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2. The biotin (strept)avidin system-principles and applications in biotechnology;Diamandis;Clin Chem,1991
3. Streptavidins with intersubunit crosslinks have enhanced stability;Reznik;Nat Biotechnol,1996
4. Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein;Aslan;Proc Natl Acad Sci USA,2005
5. A monovalent streptavidin with a single femtomolar biotin binding site;Howarth;Nat Methods,2006
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