The Impact of Metagenomics on Biocatalysis

Author:

Hogg Bethany N.1,Schnepel Christian2,Finnigan James D.3,Charnock Simon J.3,Hayes Martin A.4,Turner Nicholas J.1ORCID

Affiliation:

1. Department of Chemistry University of Manchester Manchester Institute of Biotechnology 131 Princess Street Manchester M1 7DN UK

2. School of Engineering Sciences in Chemistry, Biotechnology and Health Department of Industrial Biotechnology KTH Royal Institute of Technology AlbaNova University Center 11421 Stockholm SE

3. Prozomix Building 4 West End Ind. Estate Haltwhistle NE49 9HA UK

4. Compound Synthesis and Management Discovery Sciences Biopharmaceuticals R&D AstraZeneca Mölndal 431 50 Gothenburg SE

Abstract

AbstractIn the ever‐growing demand for sustainable ways to produce high‐value small molecules, biocatalysis has come to the forefront of greener routes to these chemicals. As such, the need to constantly find and optimise suitable biocatalysts for specific transformations has never been greater. Metagenome mining has been shown to rapidly expand the toolkit of promiscuous enzymes needed for new transformations, without requiring protein engineering steps. If protein engineering is needed, the metagenomic candidate can often provide a better starting point for engineering than a previously discovered enzyme on the open database or from literature, for instance. In this review, we highlight where metagenomics has made substantial impact on the area of biocatalysis in recent years. We review the discovery of enzymes in previously unexplored or ‘hidden’ sequence space, leading to the characterisation of enzymes with enhanced properties that originate from natural selection pressures in native environments.

Funder

Engineering and Physical Sciences Research Council

European Research Council

Publisher

Wiley

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