Retrospective study on the occurrence of Salmonella serotypes in veterinary specimens of Atlantic Canada (2012–2021)

Author:

Ojha Shivani12ORCID,Thakur Krishna K.3ORCID,Ojasanya Rasaq A.3ORCID,Saab Matthew E.23ORCID

Affiliation:

1. Department of Pathology and Microbiology Atlantic Veterinary College, University of Prince Edward Island Charlottetown Prince Edward Island Canada

2. Veterinary Diagnostic Services Laboratory Atlantic Veterinary College, University of Prince Edward Island Charlottetown Prince Edward Island Canada

3. Department of Health Management Atlantic Veterinary College, University of Prince Edward Island Charlottetown Prince Edward Island Canada

Abstract

AbstractAimThis study aimed to summarize the frequency and the antimicrobial susceptibility profiles of the Salmonella serotypes identified from the specimens of companion animals, livestock, avian, wildlife and exotic species within Atlantic Canada.Materials and MethodsThe retrospective electronic laboratory data of microbiological analyses of a selected subset of samples from 03 January 2012 to 29 December 2021 submitted from various animal species were retrieved. The frequency of Salmonella serotypes identified, and their antimicrobial susceptibility results obtained using the disk diffusion or broth method were analysed. The test results were interpreted according to the Clinical and Laboratory Standards Institute standard. The Salmonella serotypes were identified by slide agglutination (Kauffman–White‐Le‐Minor Scheme) and/or the Whole Genome Sequencing for the Salmonella in silico Serovar Typing Resource–based identification.ResultsOf the cases included in this study, 4.6% (n = 154) had at least one Salmonella isolate, corresponding to 55 different serovars. Salmonella isolation was highest from exotic animal species (n = 40, 1.20%), followed by porcine (n = 26, 0.78%), and canine (n = 23, 0.69%). Salmonella subsp. enterica serovar Typhimurium was predominant among exotic mammals, porcine and caprine samples, whereas S. Enteritidis was mostly identified in bovine and canine samples. S. Typhimurium of porcine origin was frequently resistant (>70.0%) to ampicillin. In contrast, S. Typhimurium isolates from porcine and caprine samples were susceptible (>70.0%) to florfenicol. S. Oranienburg from equine samples was susceptible to chloramphenicol, but frequently resistant (>90.0%) to azithromycin. In avian samples, S. Copenhagen was susceptible (>90.0%) to florfenicol, whereas Muenchen was frequently resistant (>90.0%) to florfenicol. S. subsp. diarizonae serovar IIIb:61:k:1,5 of ovine origin was resistant (50.0% isolates) to sulfadimethoxine. No significant changes were observed in the antibiotic resistance profiles across the study years.ConclusionsThis report provides data for surveillance studies, distribution of Salmonella serotypes and their antimicrobial resistance among veterinary specimens of Atlantic Canada.

Publisher

Wiley

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