Affiliation:
1. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, College of Bioengineering Chongqing University Chongqing China
2. Department of Hematology, Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 China
Abstract
AbstractThough magnetic iron oxide nanoparticles (IONPs) are approved for clinical use as contrast agents for MR imaging in United States and Europe, and are widely used to label cells in research, the relationship between IONPs and mesenchymal stem cells (MSCs) is not fully addressed. Here the effects of consistently appeared γ‐Fe2O3 on the lineage commitment of MSCs were studied to optimize applications of IONPs in MSCs upon verification of viability. 30 nm 10 μg/mL induced highest promotions on osteogenesis, while 30 and 50 nm of 100 μg/mL elicited most chondrogensis in 14 days, where the effects on ALP, GAG and SOX9 appeared after 7 days, while on RUNX2 came out after 10 days. γ‐Fe2O3 enhanced intracellular and extracellular Fe3+ and ROS, modulated F‐actin and decreased Lamin A of MSCs at different time scale. The disturbances of F‐actin, Lamin A or ROS altered the effects of γ‐Fe2O3 on MSC differentiation. Our results demonstrate that different size, concentration and modulation of γ‐Fe2O3 are needed in its MSC applications for bone and cartilage tissues. Furthermore, an undocumented phenomenon that the modulation of F‐actin affected the Lamin A expression in MSCs was observed.
Funder
Fundamental Research Funds for the Central Universities
National Natural Science Foundation of China
Subject
Metals and Alloys,Biomedical Engineering,Biomaterials,Ceramics and Composites